du145 (Santa Cruz Biotechnology)
Structured Review
![Figure 5. MED15 knockdown and proliferation in PCa cells. Correlation of MED15 expression with tumor proliferation markers Ki67 and PHH3 (a). Expression of MED15 in PC3 (b) and <t>DU145</t> (c) nuclear extracts by Western blot. SiRNA mediated MED15 knockdown in DU145 and PC3 cell lines by Western blot (d) and IHC (e). MTT proliferation assay of PC3 cells (f). *p < 0.05, **p < 0.01, ***p < 0.001. Similarly, proliferation assay of DU145 cells (g). *p < 0.05, **p < 0.01, ***p < 0.001. SiRNA mediated MED15 knockdown in LNCaP cell line (h). Prolif- eration assay of LNCaP cells treated with DHT (i). *p < 0.05, **p < 0.01, ***p < 0.001. ***p < 0.001, Pearson. ShRNA stable knockdown of MED15 in the DU145 cell line (j). Proliferation assay of DU145 cells with stable knockdown of MED15 compared to the scrambled control (k). *p < 0.05, **p < 0.01, ***p < 0.001. Scale bar, 10 mm. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]](https://pub-med-unpaywalled-images-cdn.bioz.com/pub_med_ids_ending_with_4838/pm24374838/pm24374838__page7_image1.jpg)
Du145, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/du145+nuclear+extract/DU+145+nuclear+extract/pm24374838-58-12-14
Average 85 stars, based on 2 article reviews
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1) Product Images from "MED15, encoding a subunit of the mediator complex, is overexpressed at high frequency in castration-resistant prostate cancer."
Article Title: MED15, encoding a subunit of the mediator complex, is overexpressed at high frequency in castration-resistant prostate cancer.
Journal: International journal of cancer
doi: 10.1002/ijc.28647
Figure Legend Snippet: Figure 5. MED15 knockdown and proliferation in PCa cells. Correlation of MED15 expression with tumor proliferation markers Ki67 and PHH3 (a). Expression of MED15 in PC3 (b) and DU145 (c) nuclear extracts by Western blot. SiRNA mediated MED15 knockdown in DU145 and PC3 cell lines by Western blot (d) and IHC (e). MTT proliferation assay of PC3 cells (f). *p < 0.05, **p < 0.01, ***p < 0.001. Similarly, proliferation assay of DU145 cells (g). *p < 0.05, **p < 0.01, ***p < 0.001. SiRNA mediated MED15 knockdown in LNCaP cell line (h). Prolif- eration assay of LNCaP cells treated with DHT (i). *p < 0.05, **p < 0.01, ***p < 0.001. ***p < 0.001, Pearson. ShRNA stable knockdown of MED15 in the DU145 cell line (j). Proliferation assay of DU145 cells with stable knockdown of MED15 compared to the scrambled control (k). *p < 0.05, **p < 0.01, ***p < 0.001. Scale bar, 10 mm. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]
Techniques Used: Knockdown, Expressing, Western Blot, Proliferation Assay, shRNA, Control
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